Web(A) Transwell migration of THP-1 macrophage in response to the cytokine secretion of ARPE-19 cells exposed to pro-oxidant conditions for 24 h was assessed after 4 h incubation. WebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically …
THP-1 In Vitro Spheroid Migration Assay (Migration) - Creative …
WebMay 1, 2024 · Effects of phorbol myristate acetate (PMA) on migration of THP-1 cells. (A) The migration ability of THP-1 cells was measured by transwell migration assay. (B) The … WebThe MCP-1 induced migration of the THP-1 monocyte cell line increased after MQ treatment in a dose dependent manner and the largest increase was observed at $0.1{\mu}M$. The level of cAMP expression decreased after a treatment with $0.1{\mu}M$ MQ. bleakrock isle complete map
Thp 1 Cell Migration Assay Rantes Millipore Bioz
WebTo this end, we disabled Ccr2 using an anti-Ccr2 antibody and performed a transwell migration assay. Blockade of Ccr2 with an anti-Ccr2 antibody appreciably diminished migration of THP-1 cells to conditioned medium (Figure 3f). These data suggest that phagocyte migration is induced through the Mcp-1-Ccr2 axis during clearance of … WebJan 1, 2024 · We also found that the induction of THP1 migration by co-culturing with LoVo-A2 was abrogated after neutralizing CCL2 (Figure (Figure6 6 B). In addition, to explore whether Hmga2 facilitated TAM infiltration via a CCL2-dependent way in vivo, we conducted the rescue experiments in mice by using the neutralizing anti-CCL2 antibody. WebJul 29, 2011 · The anti-atherosclerotic effect of cilostazol was investigated on modulating the MCP-1-induced chemotaxis of monocytes for the first time. THP-1 cells were treated with different doses of cilostazol from 0, 10, 25, 50 to 100 μM or 250 μM dibutyryl cyclic AMP (dbcAMP) for 24 h, and the chemotaxis of monocytes toward MCP-1 was analyzed … bleak setting passage and explanation