WebJul 17, 2024 · Stain the Gels with Pro-Q™ Diamond Phosphoprotein Gel Stain (Invitrogen, USA) for 2 h in the dark (see Note 14). 5. Destain the gels. Incubate the gel in 1 L of destain solution with gentle agitation for 30 min at room temperature, protected from light. Repeat this procedure two times (see Note 15). 6. Wash the gel. WebPhosphoproteins offer a unique minute-by-minute record of ongoing signal pathway events of high functional relevance to therapeutic target selection and the prediction of toxicity. Phosphorylation and dephosphorylation of structural and regulatory proteins are major intracellular control mechanisms [74].
Analysis of Protein Phosphorylation Using Phos-Tag Gels
WebPhos-tag gels are recent tools to dissect protein phosphorylation that operate by inducing a shift in the electrophoretic mobility of phosphorylated proteins compared to their nonphosphorylated counterparts. http://wolfson.huji.ac.il/purification/PDF/PAGE_SDS/Protein_Staining/PerkinElmer_PhostagGelStain.pdf#:~:text=Phos-tag%20phosphoprotein%20gel%20stains%20are%20a%20new%20family,Phos-tag%20540%20and%20Phos-tag%20300%2F460%20phosphoprotein%20gel%20stains. garching pfarrer peter
How can I do for detect the phosphoproteins by western
WebPhospho-Tag™ Phosphoprotein Destain Solution is used for phosphoprotein gel stain to reduce nonspecific binding. The destain solution is prepared with sodium acetate buffer … Web10. Discard Reagent 6, destain the gel by adding 25mL of Reagent 1 and gently agitating the gel for 15 minutes. Repeat this step once. At this stage, a green band of phosphoprotein can be visualized against a faint green background. 11. Completely destain the gel in 25mL of Reagent 7 and incubate overnight with gentle agitation. Change the ... WebNov 24, 2024 · (d) SSIIa extracted from starch granules is present as a phosphoprotein that can be dephosphorylated by APase. Conditions as in (c), except that soluble APase was used, which gives rise to the background staining and contaminants. Stromal SSIIa shown for comparison. garching near munich