Phis2.1

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August undefined, 2024

WebbpHIS-2 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … Webb1 dec. 2024 · d Growth of yeast cells on SD/-Trp-Leu-His supplemented with 100 mM 3-AT. pHIS2.1-ProGA2ox3 plus pGADT7 and pHIS2.1-ProGA2ox7 plus pGADT7 served as the negative controls.

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Webb1 mars 2024 · C. Yeast one-hybrid assay detect the specific binding ability of VvWRKY30 to W-BOX. pGADT7-empty and pHIS2.1-W-BOX, AD- VvWRKY30 and pHIS2.1-empty were … WebbProbe 2 and Probe 4 sequences as wells mutant Probe 2M and Probe 4M sequences were cloned to pHIS2.1, which drives the expression of HIS3 . GBFs were cloned to pGADT7. Yeast cells were co-transformed with a combination of the indicated plasmids or empty vector and plated onto nonselective (−Leu/−Trp) and selective ... bitcoin classic price

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WebbPhis2 1 Bait Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … WebbBait Vector Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > bait vector phis2. bait vector phis2 TaKaRa is a verified supplier TaKaRa ... bitcoinclix - earn bitcoin online

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VvWRKY30, a grape WRKY transcription factor, plays a …

Webb1.一袋培养基干粉倒入三角瓶或培养瓶中，加0.5L去离子水中溶解（琼脂冷水不溶）。 2.无调整pH值，121℃高压灭菌15min。 3.液体培养基封好口避光、室温储存备用。 4.固体 … Webb12 okt. 2024 · Moreover, a yeast one-hybrid assay was performed to verify the interaction between CnGATA20 and the CnOLE18 promoter. pHIS2.1-pro53 and pGADT7-53 (pHIS2.1-pro53+pGADT7-53) were cotransferred into yeast Y187 as a positive control, and pHIS2.1-proCnOLE18+pGADT7-53 was used as a negative control. daryl dixon winter stormWebb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used was 100 mM. daryl dixon\u0027s crossbow

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Phis2.1

Webb26 mars 2024 · The promoter sequence (1800 bp) of MdMATE8 (MDP0000175055) was inserted into the pHIS2.1 vector (Clontech, Palo Alto, CA, United States), which contained … WebbClose You have no items in your shopping cart.. Home; Plasmid; p53- His2 Model: PVT11251

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WebbPlasmid pHIS2-hsSTAM1 (1-143) from Dr. James Hurley's lab contains the insert phis2- STAM1(1-143) and is published in Structure. 2009 Mar 11. 17(3):406-16. This plasmid is … Webb15 okt. 2024 · For the protein–DNA-binding experiment, an oligonucleotide sequence containing triple tandem copies of the W-box and mW-box was synthesised, annealed, and cloned into the pHIS2.1 vector forming W-box- and mW-box-specific reporter vectors.

Webb6 feb. 2024 · Europe PMC is an archive of life sciences journal literature. Webb16 okt. 2014 · Specifically, the bait construct pHis2.1−2 × 150 was generated by cloning tandem copies of the 150-bp enhancer (2 × 150) into the MluI site of pHis2.1, a one …

WebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … WebbThe promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ...

Webb27 mars 2015 · In case of pHis2.1-Seq15 containing 4CRM-DJ1E, 50 m m 3-AT was sufficient to suppress background growth. As prey to select for TFs that bind to the …

Webb+ 5 µg pHis2.1 20 µl Note: To denature carrier DNA, heat to 95–100°C for 5 min, then cool rapidly in an ice bath. NOTE NOTE Protocol No. PT3529-1 Version No. PR7Z2445 32 3. Add competent cells and gently mix. 4. Add PEG/LiAc and gently mix. 5. Incubate at 30°C . NOTE: Mix cells every 10–15 min. www.clontech.com 50 µl 500 µl 30 min 600 µl 2.5 ml daryl dixon x beth ao3Webb24 mars 2024 · In case of 4CRM1-pHis2.1, 4CRM1mut2-pHis2.1, and 4CRM1mut5-pHis2.1, 50 mM 3-amino-1,2,4-triazole (3-AT) was sufficient to suppress background growth of yeast colonies. DNA from the TF-only prey library in pDEST_GAD424 (Mitsuda et al. 2010) was transformed into Y1HGold containing 4CRM1-pHis2.1. daryl dixon x rated romance fanfictionWebbpHIS2-hsSTAM1 (1-143) Sequences pHIS2-hsSTAM1 (1-143) Sequences (2) Addgene Sequences: Partial (1) Depositing Scientist Sequences: Partial (1) Partial Sequences … bitcoin closeWebb1 juni 2024 · When the insertion sequence is predicted as known cis-acting element , construct the pHIS2 reporter by three tandem copies of the studied elements inserted at … daryl dixon triumph chopperWebbPhis2 1 Bait Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > phis2 1 bait plasmid. phis2 1 bait plasmid TaKaRa is a ... bitcoin claim appWebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the … daryl dixon with dog funko popWebb1 sep. 2024 · 1-Methylcyclopropene counteracts ethylene promotion of fruit softening and roles of MiERF2/8 and MiPG in postharvest mangoes. ... and pHIS2-MiPG were cultivat ed on SD/-T r p/-His for 3 days, and . bitcoin clever 楽天