WebMar 24, 2024 · It utilizes human Flp-In TM cells, which genomically express a Protein A-tagged version of the human peroxisomal import receptor PEX5L. Native soluble and membrane-bound complexes containing PEX5L can thereby be isolated via a well-known affinity-based strategy. ... Flp-In™ 293 (Invitrogen), stably expressing the target protein … WebJan 6, 2024 · Firstly I inserted my target gene (pig calcium sensing receptor,pCaSR) into pcDNA 5/FRT vector, and then co-transfected pcDNA5/FRT/pCaSR and POG44 into Flp …
Can you grow HEK293 FreeStyle on plate (rather than suspension)?
WebThe Flp-In™ T‑REx™-293 cell line contains two stably, independently integrated plasmids which exhibit the following features: • The pFRT/lacZeo plasmid introduces a single FRT … WebJun 13, 2016 · The cytotoxicity test demonstrated that IC50 value of atorvastatin to inhibit the growth and/or viability of Flp-In-293/ABCB1 (2677G) cells was 5.44 ± 0.10 mM, which was significantly lower than those in Flp-In-293/ABCB1 (2677 T) (6.02 ± 0.07 mM) and Flp-In-293/ABCB1 (2677A) cells (5.95 ± 0.08 mM). ... lithonia bzl
Differential HDAC1/2 network analysis reveals a role for
WebNov 1, 2010 · Wild-type and mutant BCRP-expressing HEK-293 or Flp-In-293 cells and the vector control cells were plated in four-chamber polysterene vessels on glass slides (Falcon; BD Biosciences, Bedford, MA) at ∼2 × 10 5 cells/well. Cells were grown to 80% confluence and washed twice with PBS at room temperature. Cells were then fixed with 4% ... WebDec 5, 2013 · By using the conditional promoter in the Flp-In-Trex vector, functional expression of Npt2A was achieved by doxycycline induction in HEK293 cells. The EGFP tagged and non-tagged, inducible stable hNpt2A-HEK293 cell lines afforded development of a robust phosphate uptake assay mediated by hNpt2A, which can be used to screen … WebB , Flp-In T-Rex 293 cell line (Flp-In 293) engineered to stably express NUBP2 was induced overnight with 1 μg/ml doxycycline. Affinity purified NUBP2 and associated proteins were identified by bottom-up proteomics. imtiaz online grocery contact number