Ctffind4.1
WebMar 15, 2024 · MotionCor2.1 was used to perform the frame-based motion-correction algorithm to generate drift-corrected micrographs for further processing and CTFFIND4.1 provided the estimation of the contrast transfer function (CTF) parameters (55, 56). WebJob: CTFFIND4 (Wrapper) Job: Gctf (Wrapper) (Legacy) Exposure Curation Particle Picking Extraction Deep Picking Particle Curation 3D Reconstruction 3D Refinement …
Ctffind4.1
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WebMar 2, 2024 · The CTF parameters were estimated using CTFFIND4 . A total of 1 120 554 particles were automatically picked by blob-picker and extracted from the five datasets with default parameters. Two rounds of 2D classification were performed, where 30S and 70S particles are removed from the dataset. 900 883 particles were selected and used for the ... WebJun 25, 2024 · Our new normal has forced exponential data growth not just in our data centers, but also from remote workforces and in SaaS productivity platforms, such as …
WebApr 1, 2013 · 11total downloads. Last upload: 2 years and 2 months ago. Installers. linux-64v4.1.13. conda install. To install this package run one of the following:conda install -c … WebDec 20, 2024 · error message in Ctffind4.1 #559 Closed ananyachatterjeeiiserkolkata opened this issue on Dec 20, 2024 · 1 comment ananyachatterjeeiiserkolkata …
WebCTFFIND is a widely-used program for the estimation of objective lens defocus parameters from transmission electron micrographs. Defocus parameters are estimated by fitting a … WebI’ve found that CTFFIND4.1.5 works better/more robustly than earlier versions of CTFFIND4. Whether CTFFIND4.1 works better or worse than GCTF seems to be dataset dependent. As of 7/17/19 I prefer GCTF in Relion but not “Relion’s implementation of GCTF” as it can not run on GPUs. So it is something to play around with.
WebAug 13, 2015 · CTFFIND is a widely-used program for the estimation of objective lens defocus parameters from transmission electron micrographs. Defocus parameters are estimated by fitting a model of the...
WebAbsolute path to the CTFFIND4 executable. Needs version at least above 4.1.13. gctf_exe. Absolute path to the GCTF executable. I wouldn’t use it because it rarely works but it seems a version of 1.06 sometimes doesn’t crash. exec_dir. Directory for subTOM executables. Memory Options mem_free. The amount of memory the job requires for alignment. how to restrict download from google drivenortheastern on campus jobsWebApr 11, 2024 · Section snippets Structural organization of the junctional complex. To characterize the spectrin-actin junctional complex, we isolated the membrane skeleton from Sus scrofa domestica red blood cells (Figure S1). Through the optimization of cryo-EM sample preparation, we obtained images of a well-dispersed junctional complex (Figure … northeastern one driveWebNov 16, 2024 · Image processing was performed in RELION4 , unless otherwise specified. Dose-fractionated movies were motion-corrected with RELION’s implementation of the MotionCor2 algorithm , and contrast transfer function (CTF) parameters were estimated using CTFFIND4.1 (). After 2D classification in cryoSPARC v3.3.1 , 235,444 particles … northeastern online graduate programsWebApr 1, 2013 · linux-64v4.1.13 conda install To install this package run one of the following:conda install -c gregvonkuster ctffind4 Description By data scientists, for data … how to restrict in app purchasesWebJan 11, 2024 · Image drift correction was performed using Relion’s own implementation and whole micrograph CTF estimation was performed using the program CTFFIND4.1.10 in … how to restrict jtextfield to numbers onlyWebDec 27, 2024 · It's possible to use CTFFIND4 installed separately from cisTEM by defining CTFFIND4_HOME variable in scipion.conf. Setting this value will have the priority over Ctffind inside cisTEM. A complete list of tests can be seen by executing scipion test --show --grep cistem Supported versions 1.0.0-beta (cistem) 4.1.13, 4.1.14 (ctffind4) Protocols northeastern oklahoma state