http://dzyh.szu.edu.cn/lab/orders/product/80040053271592 WebApr 13, 2024 · RBCs were lysed using ACK Lysis Buffer (Lonza 10-548E) and the blood was further diluted at 1:100 ratio in the cell staining buffer (Biolegend, 420241).

FOXP3 Fix/Perm Buffer Set BioLegend 421403 product …

WebFeb 22, 2024 · Then, cells were washed twice with cold BioLegend cell staining buffer (420241) before resuspension in Annexin V Binding Buffer (422201) at a concentration of 10 6 cells/mL. FITC Annexin V and 7-aminoactinomycin D (AAD; 420401) were added successively. DNA content was analyzed using 7-AAD. Flow cytometry was performed … WebRequest Bulk Quote. Description. Cell Staining Buffer is an antibody diluent and cell wash buffer optimized for use in immunofluorescent staining of viable or fixed single cell … solihull breast clinic

RBC Lysis Buffer (10X) - punchout.biolegend.com

WebImmunohistochemistry (IHC) Staining of Tissue Microarray. ... USA) at room temperature for 30 mins. After dark incubation, cells were washed with cell staining buffer (420,201, Biolegend, San Diego, USA), and then incubated with Fc receptor blocking solution (422,302, Biolegend, San Diego, USA) in dark, room temperature for 10 mins. WebRBC Lysis Buffer (10X) - Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. WebWash cells twice with cold BioLegend cell staining buffer (cat # 420241) and then resuspend cells in Annexin V Binding Buffer (cat # 422201) at a concentration of 1x10 6 cells/ml. 2. Transfer 100 µl of cell suspension in 5 ml test tube. 3. Add 5 µl of fluorochrome conjugated Annexin V. 4. Add 10 µl of PI solution (cat # 421301) or 7-AAD (cat ... small baking sheets